Post-translational Modification of .ALPHA.B-Crystallin of Normal Human Lens.
نویسندگان
چکیده
منابع مشابه
Effect of a single AGE modification on the structure and chaperone activity of human alphaB-crystallin.
During aging, human lens proteins undergo several post-translational modifications, one of which is glycation. This process leads to the formation of advanced glycation end products (AGEs) which accumulate with time possibly leading to the formation of cataract. alphaB-Crystallin, a predominant protein in the lens, is a member of the small heat shock proteins (sHSPs) which are a ubiquitous clas...
متن کاملEffect of deamidation of asparagine 146 on functional and structural properties of human lens alphaB-crystallin.
PURPOSE To elucidate the effect of deamidation on the structural and functional properties of human alphaB-crystallin. METHODS Site-directed mutagenesis was used to generate three deamidated mutants of alphaB-crystallin: N78D, N146D, and N78D/N146D. The mutations were confirmed by DNA sequencing and matrix-assisted desorption ionization-time of flight (MALDI-TOF) mass spectrometry. Recombinan...
متن کاملExistence of deamidated alphaB-crystallin fragments in normal and cataractous human lenses.
PURPOSE The aims of this study were to characterize lens crystallin fragments having a molecular mass of <10 kDa, isolated by solubilization in trichloroacetic acid, in order to identify cleavage sites in the parent crystallins for their origin and determine post-translational modifications in the fragments. METHODS The water-soluble (WS) and water-insoluble (WI) protein fractions were isolat...
متن کاملMorphological characterization of the AlphaA- and AlphaB-crystallin double knockout mouse lens
BACKGROUND One approach to resolving some of the in vivo functions of alpha-crystallin is to generate animal models where one or both of the alpha-crystallin gene products have been eliminated. In the single alpha-crystallin knockout mice, the remaining alpha-crystallin may fully or partially compensate for some of the functions of the missing protein, especially in the lens, where both alpha A...
متن کاملAllosteric post-translational modification codes.
Post-translational modifications (PTMs) have been recognized to impact protein function in two ways: (i) orthosterically, via direct recognition by protein domains or through interference with binding; and (ii) allosterically, via conformational changes induced at the functional sites. Because different chemical types of PTMs elicit different structural alterations, the effects of combinatorial...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2000
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.23.226